![]() The AMP-Glo™ Assay is well suited for monitoring AMP produced in biochemical reactions catalyzed by enzymes that do not use ATP as a substrate, such as cAMP-dependent phosphodiesterases (PDE) and bacterial DNA ligases. Two reagents are supplied: one to terminate the AMP-generating enzymatic reaction, remove ATP and convert AMP produced into ADP, and a second reagent to convert ADP to ATP, which is used to generate a luminescence in a luciferase reaction. The assay can be used to determine the AMP produced either in the presence or absence of ATP as a substrate. cAMP is a derivative of adenosine triphosphate (ATP) and used for intracellular signal transduction in many different organisms, conveying the cAMP-dependent pathway. Restriction endonucleases, Phusion High-Fidelity DNA polymerase, T4 DNA ligase and Gibson Assembly Master Mix were obtained. The stable luminescent signal eliminates the need for an injector-equipped luminometer and allows batch-mode processing of multiple plates. Cyclic adenosine monophosphate (cAMP, cyclic AMP, or 3',5'-cyclic adenosine monophosphate) is a second messenger, or cellular signal occurring within cells, that is important in many biological processes. CAY10499 and cyclic AMP assay kits were obtained from Cayman Chemical. ![]() The assay quantitatively monitors AMP concentration in a biochemical reaction in a wide range of plates, including high-throughput formats. It can be used to measure the activity of a broad range of enzymes, such as cyclic AMP-specific phosphodiesterases, aminoacyl-tRNA synthetases, DNA ligases and ubiquitin ligases or enzymes modulated by AMP. The AMP-Glo™ Assay is a homogeneous assay that generates a luminescent signal from any biochemical reaction that produces AMP.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |